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Comparative Efficacy and Safety of Bismuth Quadruple and Concomitant Therapy for Empirical Helicobacter pylori Eradication: A Meta-Analysis of Randomized Clinical Trials
Qiu Ju Lyu,
Qiang Hong Pu
Issue:
Volume 8, Issue 4, December 2022
Pages:
113-118
Received:
7 September 2022
Accepted:
29 September 2022
Published:
11 October 2022
DOI:
10.11648/j.bs.20220804.11
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Abstract: Aims: To compare the efficacy and safety of bismuth quadruple therapy with concomitant therapy in the empirical eradication of Helicobacter pylori (H. pylori) infection. Methods: Such databases as PubMed, Embase and the Cochrane Library and Chinese databases (China National Knowledge Infrastructure (CNKI), Wanfang Data and CBM), were searched for relevant randomized controlled trials up to February 2020. Studies were included if they assessed the efficacy and safety of bismuth quadruple therapy versus concomitant therapy in H. pylori eradication. Statistical analysis was performed with RevMan software 5.3. Results: Four studies with 616 patients were evaluated in this meta-analysis. The H. pylori eradication rate of bismuth quadruple therapy was similar to that of concomitant therapy (intention-to-treat analysis: pooled eradication rates, 85.5% vs 80.7%; odds ratio [OR], 1.42; 95%confidence interval (CI): [0.92–2.18]; P>0.05). The incidence of adverse events in bismuth quadruple therapy was lower than those in concomitant therapy (pooled incidence, 18.3% vs 25.9%; OR, 0.62, 95%CI: [0.41–0.92]; P<0.05). Conclusions: Bismuth quadruple therapy had the same efficacy to concomitant therapy in H. pylori eradication, and bismuth quadruple therapy was possibly better tolerated than concomitant therapy. Therefore, bismuth quadruple therapy and concomitant therapy should be equally recommended as empirical regimens in H. pylori eradication.
Abstract: Aims: To compare the efficacy and safety of bismuth quadruple therapy with concomitant therapy in the empirical eradication of Helicobacter pylori (H. pylori) infection. Methods: Such databases as PubMed, Embase and the Cochrane Library and Chinese databases (China National Knowledge Infrastructure (CNKI), Wanfang Data and CBM), were searched for r...
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Effect of Tai Chi and Yoga Training on Oxidative Status, Physical Performance, and Cognitive Function of Military Personnel
Mo Mo Than,
Khin Nwe Kyi,
Khine Kyaw Oo,
Phyo Thaw Htun,
Yan Naing Soe,
Paing Khant Kyaw,
Kaung Su Lin,
Min Thein,
Ye Myat Kyaw,
Khun Zaw,
Zaw Min Thein,
Zaw Min Htut,
Kyaw Myo Htun,
Thet Naing,
Ko Ko Lwin
Issue:
Volume 8, Issue 4, December 2022
Pages:
119-125
Received:
20 September 2022
Accepted:
8 October 2022
Published:
18 October 2022
DOI:
10.11648/j.bs.20220804.12
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Abstract: Yoga, a long-established Indian philosophical system, tries to bring harmony and wellness to the physical, mental, and emotional dimensions. Tai Chi is a traditional Chinese exercise of moderate intensity. They are widely used in modern society to reduce oxidative biological alterations and enhance physical and mental performance. The main objective was to evaluate the effect of Tai Chi and Yoga exercises on oxidative stress measures, physical performance, and cognitive function of military personnel. It was a field-based pragmatic randomized control study. Military medical officers who were taking a refresher course for Military Medical Training from February to March 2020 comprised mainly 81 voluntary participants. They were divided into three groups in equal chance, with each receiving six weeks of regular, Tai Chi, and Yoga training. Blood samples were obtained before and following the six-week interventions, and measurements of the serum nitric oxide (NO) and oxidative stress index (OSI) were taken. Then assessments of cognitive function and physical fitness were conducted. Significant reductions of OSI from 0.61 ± 0.14 AU to 0.32 ± 0.12 AU (p < 0.01) was found in the Tai Chi group and the effect of Tai Chi on OSI level reduction was found more prominently over Yoga effects, although there were no remarkable differences in NO level among them. For physical fitness test, parameters of Yoga group (Body fat percent: 20.87 ± 3.62 to 19.84 ± 3.53; Sit and Reach test: 2.00 ± 1.78 to 2.89 ± 1.70 (times/minute); Crunch test: 34.07 ± 8.94 to 37.21 ± 11.89 (times/minute) were improved significantly (p < 0.05). In the Tai Chi group, improvements were seen in Sit and Reach test: 2.11 ± 1.66 to 2.89 ± 2.04 (times/minute); and Crunch test: 31.81 ± 7.49 to 37.85 ± 8.83 (times/minute), (p < 0.05). As for the cognitive function test, there were no significant changes in all groups. According to the study’s outcomes, Yoga training is generally superior to Tai Chi training in terms of areas of physical fitness testing. It can be concluded that a six-week Tai Chi and Yoga exercise program has beneficial effects on antioxidant capacity and oxidative stress measurements. While the practice of Tai Chi generates a more intense antioxidant impact, Yoga produces a greater improvement in physical fitness effect.
Abstract: Yoga, a long-established Indian philosophical system, tries to bring harmony and wellness to the physical, mental, and emotional dimensions. Tai Chi is a traditional Chinese exercise of moderate intensity. They are widely used in modern society to reduce oxidative biological alterations and enhance physical and mental performance. The main objectiv...
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Discussion on Molecular Mechanism of Siwei Xiaoliuyin in Treating Glioma Based on Network Pharmacology and Molecular Docking
Biaogang Han,
Xiaohong Wu,
Xiaopei Zhang,
Shihua Liu,
Yongqing Shen,
Aixia Sui
Issue:
Volume 8, Issue 4, December 2022
Pages:
126-137
Received:
4 November 2022
Accepted:
17 November 2022
Published:
29 November 2022
DOI:
10.11648/j.bs.20220804.13
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Abstract: Background. Siwei Xiaoliuyin, a traditional Chinese medicine, is effective in treating glioma, but its molecular mechanism of action is still unclear. In this paper, we will explore the molecular mechanism of Siwei Xiaoliuyin in the treatment of glioma through network pharmacology. Methods. The potential active components and molecular targets of Siwei Xiaoliuyin were collected through the pharmacological database and analysis platform of traditional Chinese medicine system and TCMID database; glioma-related target genes were obtained through the GenCards database, OMIM database and Disgenet database; the intersection of drug action targets and disease genes was extracted using R software, and Venn diagram was drawn; the key targets were imported into the String database to construct a protein interaction network; the key targets were imported into R software using clusterProfiler for GO and KEGG enrichment analysis; the main components of Siwei Xiaoliuyin were molecularly docked with the Hub gene by AutoDock Vina technology. Results. Siwei Xiaoliuyin consists of four components, which are Curcuma zedoaria, Tianlong, Solanum nigrum and Smilax glabra and a total of 26 potential active components and 56 targets were identified from it, 5750 glioma-related genes and 47 key target genes crossed between Siwei Xiaoliuyin and glioma. The results of enrichment analysis showed that GO entries involved fatty acid metabolic processes, response to steroid hormones and other processes. KEGG analysis identified key genes mainly enriched in PI3K-Akt signaling pathway, estrogen signaling pathway and HIF-1 signaling pathway, etc. The results of molecular docking showed that Diosgenin, the main component of Siwei Xiaoliuyin, docked well with the AHR gene. Conclusions. Through network pharmacology prediction, Siwei Xiaoliuyin may regulate multiple signaling pathways such as PI3K-Akt, estrogen and HIF-1 through multiple targets EGFR, ESR1, VEGFA, AHR and AR, thus affecting the function of multiple cells and playing an important role in the treatment of glioma.
Abstract: Background. Siwei Xiaoliuyin, a traditional Chinese medicine, is effective in treating glioma, but its molecular mechanism of action is still unclear. In this paper, we will explore the molecular mechanism of Siwei Xiaoliuyin in the treatment of glioma through network pharmacology. Methods. The potential active components and molecular targets of S...
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Early Diagnosis of PCT Combined with CRP in Patients with Infection After Lumbar Internal Fixation
Jieshi Wu,
Quanming Zhao,
Rui Luo,
Yankun Li,
Facai Xu,
Ruisheng Xu
Issue:
Volume 8, Issue 4, December 2022
Pages:
138-143
Received:
19 November 2022
Accepted:
2 December 2022
Published:
8 December 2022
DOI:
10.11648/j.bs.20220804.14
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Abstract: Infection is a serious complication after lumbar internal fixation, and it is the focus of current spinal surgery research. Procalcitonin is sensitive and specific biomarker for identifying bacterial infection, but whether it can be used as a detection index for the early diagnosis of infection after lumbar internal fixation requires further research. Objective: To investigate the clinical significance of procalcitonin (PCT) combined with C-reactive protein (CRP) detection in the early diagnosis of infection after lumbar internal fixation. Methods: Patients who underwent lumbar internal fixation in our hospital from August 2019 to December 2021 were retrospectively studied. According to the results of wound secretion culture, they were divided into an infection group and a noninfection group. Venous blood was collected at different time points after the operation to detect and analyse serum PCT and serum CRP. The sensitivity and specificity of combined CRP and PCT for diagnosing surgical site infection were calculated. Results: There was no significant difference in preoperative indicators among all patients, and postoperative PCT and CRP indicators were significantly higher than those before surgery. The sensitivity of PCT combined with CRP to detect early postoperative infection was higher than that of single use. Conclusion: The combined detection of serum PCT and CRP can be used as a diagnostic index for early infection after lumbar internal fixation and has value in clinical application.
Abstract: Infection is a serious complication after lumbar internal fixation, and it is the focus of current spinal surgery research. Procalcitonin is sensitive and specific biomarker for identifying bacterial infection, but whether it can be used as a detection index for the early diagnosis of infection after lumbar internal fixation requires further resear...
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Identification of Long Noncoding RNA Recognized by RNA-Binding Protein TLS/FUS: Purification of RNAs by Affinity Chromatography of GST-TLS
Naomi Ueda,
Ryoma Yoneda,
Riki Kurokawa
Issue:
Volume 8, Issue 4, December 2022
Pages:
144-156
Received:
1 November 2022
Accepted:
25 November 2022
Published:
15 December 2022
DOI:
10.11648/j.bs.20220804.15
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Abstract: RNA binding protein TLS/FUS binds promoter-associated noncoding RNA-D (pncRNA-D), long noncoding RNA (lncRNA), and inhibits histone acetyltransferase (HAT) activity in cellular nuclei to repress the cyclin-D1 gene transcription. TLS is expressed in neuronal cells and plays pivotal roles in neuronal develop and function. Its precipitation in motor neurons is supposed to cause amyotrophic lateral sclerosis (ALS) one of neurodegenerative diseases. Our preliminary experiments indicate that pncRNA-D should repress the phase separation and the resultant precipitation of TLS in biochemical conditions. This implies that pncRNA-D be a seed for effective drug against ALS. It is conceivable that a pool of lncRNAs bound to TLS should be a competent library screening for an ALS drug. Then, we decided to search for more lncRNAs to regulate phase separation and precipitation. In this manuscript, we develop a simple and swift technology of the affinity purification of unidentified RNAs from HeLa cell total RNA using bacterially expressed glutathione S-transferase-tagged-TLS (GST-TLS). Screening of the GST-TLS bound RNA has been performed with a human lncRNA microarray using a fluorescent dye, the cy3-labeled bound RNA as a probe. 1728 lncRNAs of more than two-fold increase at the fluorescent signals have been identified, compared to those of the input HeLa cell total RNA. The top 25 lncRNAs from the 1728 lncRNAs were expressed at more than 12-fold induction. Tentatively, the top four putative lncRNAs were employed for further analysis. Then, these lncRNAs have been shown to have specific binding to GST-TLS and also cellular TLS. The precipitation based experiment to detect phase separation has shown that these lncRNAs inhibit the phase separation-induced precipitation which is dissolved in 1, 6-hexanediol (1,6-HD). There is no significant sequence homology over these lncRNAs, although a consensus conformation of these lncRNAs is the loops and stems structure based upon the predicted secondary structures of the top 25 lncRNAs selected. These data confirm that the GST-TLS based affinity purification of RNA bound to TLS works well to provide the novel lncRNAs specific to TLS. The method to identify novel lncRNAs recognized by TLS provides a profitable technique for initiating the biology of TLS-bound lncRNAs in cellular programs.
Abstract: RNA binding protein TLS/FUS binds promoter-associated noncoding RNA-D (pncRNA-D), long noncoding RNA (lncRNA), and inhibits histone acetyltransferase (HAT) activity in cellular nuclei to repress the cyclin-D1 gene transcription. TLS is expressed in neuronal cells and plays pivotal roles in neuronal develop and function. Its precipitation in motor n...
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